FOR DILUTING AND MIXING GROWDEX
can easily be diluted with e.g. cell culture media, PBS, or
for use in cell-based assays. Concentrations of 0.2-1.0 % are commonly used for
cell culture applications. The optimal concentration will depend on the cell
type being used, e.g. 0.5 % for WA07 human embryonic stem cells and 0.8-1.0%
and HepG2 hepatic cells. Refer to ‘GrowDex Instructions for use’ for
more examples and also to other GrowDex application
The viscosity of GrowDex is
diluting the product to a less viscous state.
The recommended procedure for the
dilution and mixing of GrowDex is as follows:
required amount of diluent, without cells, into a test tube. Take into account
the volume of the cell suspension that will be added in Step 5
ensure the correct final volume and concentration.
directly from the syringe provided or pipette the required amount into the test
tube containing the diluent. Graduations on the syringe indicate the volume
dispensed or alternatively GrowDex may be weighed.
GrowDex and diluent by pipetting up and down for a minimum of 90 seconds until
a homogenous solution is achieved by visual inspection.
bubble formation by keeping the pipette tip submerged in the solution
throughout the mixing process.
cell suspension to the test tube slowly and stir carefully with the pipette tip
to avoid damaging the cells until the cells are evenly dispersed throughout the
GrowDex/cell mix is now ready to use.
3D CELL CULTURE EXPERIMENTS
required = 0.5 % / Final volume = 1 mL
the needed amounts of stock GrowDex (1.5%) and cell culture medium. NOTE: Take
into account the volume in which the cells are seeded into GrowDex
of stock GrowDex (1.5%)= (Final volume
of assay × required GrowDex concentration (%))/(1.5%)
of culture media=Final volume of assay -Volume of stock GrowDex - Volume of
medium into a test tube.
and mix by pipetting up until the solution is homogenous by visual inspection.
tips are recommended for this procedure. More practical tips for
the use of GrowDex can be found in Section 3 in ‘GrowDex Instructions for use’.
B.Pre-diluted GrowDex without cells
can be stored for 7 days at 2-8°C if no unstable components are present in the
suspension to the diluted GrowDex slowly and stir carefully using the pipette
tip to evenly disperse the cells.
ready for use at a working concentration of
GrowDex, diluent and cell suspension required for the preparation of 1 ml of
diluted GrowDex for a variety of final working concentrations.
To order visit our online store at www.growdex.com, or contact us at firstname.lastname@example.org for questions or a quotation.