GrowDex® - The cellulose based hydrogel for 3D cell culture

3D cell culture hydrogel

​​3D cell culture – more natural results

3D cell culture is an in vitro technology for advanced cell culture applications. Cells grown in 3D mimic more closely natural tissues and organs than cells grown in 2D. 3D cell culture techniques enable e.g. development of cell based drug and chemical tests and discovery of models and treatment for serious diseases.

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GrowDex® 3D cell culture hydrogel with outstanding unique features

GrowDex® is a flexible 3D cell culture hydrogel providing a number of benefits for 3D cell culture applications:

  • High biocompability with human cells and tissues without any human or animal nature of constituents
  • Simple to use, handle and modify
    • One component system tunable with e.g. water or media
    • Can be stored in room temperature
    • No crosslinking step needed
  • Good viability and functionality of cells
  • The hydrogel can be completely removed by enzymatic degradation while retaining the 3D spheroid structure
  • Uniform quality for reliable and repeatable result

 
GrowDex® is an ideal hydrogel and it has several favorable material properties:

  • Non-animal based
  • Highly viscous i.e. high gel force but tunable
  • Dispense by pipette - manual or automated
  • High water retention
  • Tolerates salts, temperatures and pH
  • Not autofluorescent
  • Delivery pH 6-8

3D cell culture with flexibility

GrowDex® is a cellulose nanofibril hydrogel with an ultrastructure and mechanical properties that can be tuned to fulfill the requirements of different cell types. GrowDex® is an hydrogel that forms a supporting matrix around the cells, and supports 3D cell growth. It resembles extracellular matrix (ECM), since the fibril size mimics natural ECM fibers. GrowDex® allows diffusion of small molecules, such as nutrients and oxygen. GrowDex®-based hydrogel cell culture can be further optimized by adding bioactive components to the scaffold. The hydrogel can also be completely deactivated by enzyme treatment.

GrowDex® has been used as a 3D cell culture matrix for different types of cells, such as WA07 and iPS(IMR90)-4 stem cell lines, and HepG2, HepaRG and ARPE-19 cell lines.

GrowDex® is available in 5 ml vials. One vial gives GrowDex® hydrogel for 100-200 wells on a 96 well plate.

For ordering please fill in the ordering form.

3D cell culture hydrogel
 
Figure 1. iPS-DF19-9-7T cells were cultured in 0.5% GrowDex® for the indicated days. Cell viability is shown by live/dead staining. Pluripotency is shown by the expression of OCT4 in 3D. Images are provided by Yan-Ru Lou et al., University of Helsinki, Faculty of Pharmacy, Finland.

 
For more information please email GrowDex@upm.com »

3D cell culture references

Meet us at the following events


ELRIG's Research & Innovation, 29-30 March 2017 Cambridge, UK. Conference site »

Advances in Cell Based Screening, 10-11 May 2017, Gothenburg, Sweden Conference site »

ISSCR 2017 Annual Meeting 2017, 14-17 June 2017, Boston, US. Conference site »

EMBEC`17&NBC´16 conference, 11-15 June 2017, Tampere, Finland. Conference site »

References

SCIENTIFIC ARTICLES

Paukkonen H. et al. Hydrophobin-nanofibrillated cellulose stabilized emulsions for encapsulation and release of BCS class II drugs. European Journal of Pharmaceutical Sciences 2017;100: 238-248. Read more »

Lou Y.-R, et al. Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells. Scientific Reports 2015; 5, 13635. Read more »

Malinen MM, et al. Differentiation of liver progenitor cell line to functional organotypic cultures in 3D Nanofibrillar cellulose and hyaluronan-gelatin hydrogels. Biomaterials 2014;35:5110-5121. Read more »

Lou Y.-R. et al., 2014. The use of Nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells, Stem cell and development. Vol. 23:4, pp. 380–392. Read more »

Laurén P. et al. Technetium-99m-labeled nanofibrillar cellulose hydrogel for in vivo drug release. Eur J Pharm Sci. 2014 Dec 18;65:79-88 Read more »

Bhattacharya M. et al., 2012, Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture, Journal of Controlled Release 164:3, pp. 291–298. Read more »

DOCTORAL DISSERTATIONS

Liisa Kanninen, Bioinspired matrices for in vitro hepatic differentiation, University of Helsinki, Finland, 2016. Read more »

Melina Malinen, Development of organotypic liver cell cultures in three-dimensional biomaterial hydrogels, University of Helsinki, Finland, 2014, Read more »  

Ruzica Kolakovic, Nanofibrillar Cellulose in Drug Delivery, University of Helsinki, Finland, 2013, Read more »

CONFERENCE POSTERS

Lauri Paasonen, 3D cell culture, assay automation and 3D printing demonstrated with a novel xeno-free hydrogel derived from wood, ELRIG Drug Discovery 2016. Read more »

Technical brochure

Order GrowDex brochure here »