3D cell culture with flexibility
GrowDex® is a cellulose nanofibril hydrogel with an ultrastructure and mechanical properties that can be tuned to fulfill the requirements of different cell types. GrowDex® is an hydrogel that forms a supporting matrix around the cells, and supports 3D cell growth. It resembles extracellular matrix (ECM), since the fibril size mimics natural ECM fibers. GrowDex® allows diffusion of small molecules, such as nutrients and oxygen. GrowDex®-based hydrogel cell culture can be further optimized by adding bioactive components to the scaffold. The hydrogel can also be completely deactivated by enzyme treatment.
GrowDex® has been used as a 3D cell culture matrix for different types of cells, such as WA07 and iPS(IMR90)-4 stem cell lines, and HepG2, HepaRG and ARPE-19 cell lines.
Figure 1. iPS-DF19-9-7T cells were cultured in 0.5% GrowDex® for the indicated days. Cell viability is shown by live/dead staining. Pluripotency is shown by the expression of OCT4 in 3D. Images are provided by Yan-Ru Lou et al., University of Helsinki, Faculty of Pharmacy, Finland.
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